Serena Williams has lost her bid to retain her Olympics Singles title at Rio de Janeiro.
The World number one was beaten in two straight sets by Ukrainian Elina Svitolina 4-6 3-6 in the round three of the tournament.
Svitolina, ranked world number 20, even broke her when she was serving 3-5 to seal the second set.
Serena, who played out of sort, has now joined her sister, Venus who lost in the first round.
Both sisters also lost out in the doubles to complete a very disappointing Rio Olympics.
Among the players who made the fourth round are American Madison Keys, Johanna Konta of Britain and the German left-hander Angelique Kerber.
Others are Monica Puig from Puerto Rico, Laura Siegemund from Germany, who knocked out the Belgian Kirsten Flipkens, who had shocked Venus Williams in the first round.
Petra Kvitova from Czech Republic is still in the race for singles gold, just like Daria Kastkina from Russia, who knocked out Sara Errani, the Italian.